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The protein content of the samples was determined by the Bradford method 51 adapted to microplate 52

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NF-B activation allows tumor cells to evade growth-inhibitory signals, stimulate angiogenesis, invade tissues, metastasize, and replicate indefinitely ( Under the combined influence of hypoxia and ID, tumor cells adjust metabolic reprogramming-related target genes via the HIF-1 pathway, promoting cancer progression ( 6 The role of ID and ferroptosis in tumors 6.1 Ferroptosis mechanism Ferroptosis is a form of iron-dependent, non-apoptotic cell death marked by the buildup of lipid peroxides, which are catalyzed by iron

Supplementation should be initiated before or during periods of anticipated stress to provide the necessary physiological support