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One exception is the analysis of fluorescent protein substrates such as rxYFP or roGFP2, which allow a direct continuous detection of the reversible glutaredoxin-dependent protein disulfide reduction and formation in vitro 14,15,31,32,33,34,35

While several elegant methods have so far been developed for GST activity assay [5, 6], these methods are limited by the expensive materials, sophisticated instruments, or their time-consuming experimental procedures

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