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The crude material was purified by Isolera flash column chromatography on silica via gradient elution, from 100% dichloromethane to 15% methanol in dichloromethane to produce CAM-S-CAM- d 15 TPP (9.1 mg, 27%) as a pale yellow fluffy/foamy solid

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Unresolved questions It remains to be established whether CNS symptoms reported in some patients with gamma-glutamylcysteine synthetase are related to the enzyme defect or not

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